Proteintech, 11806-1-AP, CAPZA1 Polyclonal antibody

Size: 20ul / 150ul The CAPZA1 (11806-1-AP) by Proteintech is a Polyclonal antibody targeting CAPZA1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 11806-1-AP targets CAPZA1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IP detected in: SH-SY5Y cells Positive IHC detected in: human ovarian cancer, human ovary tumor tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2267 Product name: Recombinant human CAPZA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-286 aa of BC000144 Sequence: MADFDDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMDQFTPVKIEGYEDQVLITEHGDLGNSRFLDPRNKISFKFDHLRKEASDPQPEEADGGLKSWRESCDSALRAYVKDHYSNGFCTVYAKTIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFTITPPTAQVVGVLKIQVHYYEDGNVQLVSHKDVQDSLTVSNEAQTAKEFIKIIENAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA Predict reactive species Full Name: capping protein (actin filament) muscle Z-line, alpha 1 Calculated Molecular Weight: 286 aa, 33 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC000144 Gene Symbol: CAPZA1 Gene ID (NCBI): 829 RRID: AB_2070037 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P52907 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.