Proteintech, 11820-1-AP, SDC4 Polyclonal antibody

Size: 20ul / 150ul The SDC4 (11820-1-AP) by Proteintech is a Polyclonal antibody targeting SDC4 in WB, IHC, ELISA applications with reactivity to human, mouse samples 11820-1-AP targets SDC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells Positive IHC detected in: mouse skeletal muscle tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SDC4, also known as syndecan 4, is a cell surface proteoglycan that bears heparan sulfate, which is involved in normal wound healing. It has been reported that SDC4 may be associated with keratinocyte proliferation and differentiation in the epidermis. SDC4 also plays a key role in angiogenesis and inflammation. The predicted MW of SDC4 is 22 kDa, and 11820-1-AP antibody recognizes a protein around 24-26 kDa and 48-52 kDa (homodimer), which is similar to the papers.(PMID: 20639201, 21949132; 18638267; 30165731 ) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2388 Product name: Recombinant human SDC4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC030805 Sequence: MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA Predict reactive species Full Name: syndecan 4 Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 22-26 kDa GenBank Accession Number: BC030805 Gene Symbol: SDC4 Gene ID (NCBI): 6385 RRID: AB_2877797 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P31431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.