Proteintech, 11823-1-AP, CIB1 Polyclonal antibody

Size: 20ul / 150ul The CIB1 (11823-1-AP) by Proteintech is a Polyclonal antibody targeting CIB1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 11823-1-AP targets CIB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human liver tissue, HepG2 cells, rat testis tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: human breast cancer tissue, human testis tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2391 Product name: Recombinant human CIB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-191 aa of BC000846 Sequence: MGGSGSRLSKELLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSLPELKANPFKERICRVFSTSPAKDSLSFEDFLDLLSVFSDTATPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGEDTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL Predict reactive species Full Name: calcium and integrin binding 1 (calmyrin) Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 22 kDa GenBank Accession Number: BC000846 Gene Symbol: CIB1 Gene ID (NCBI): 10519 RRID: AB_2079679 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99828 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.