Proteintech, 11852-1-AP, GIMAP4 Polyclonal antibody

Size: 20ul / 150ul The GIMAP4 (11852-1-AP) by Proteintech is a Polyclonal antibody targeting GIMAP4 in WB, IHC, IP, ELISA applications with reactivity to human samples 11852-1-AP targets GIMAP4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells Positive IP detected in: Jurkat cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GIMAP4, also named as hIAN1 or HIMAP4, is a member of GIMAP family. Among GIMAP family, GIMAP4 is the only member reported to have true GTPase activity, and its transcription is found to be differentially regulated during early human CD4+ T helper (Th) lymphocyte differentiation. GIMAP4 has been previously connected mainly with T- and B-cell development and survival and T-cell apoptosis. The MW of this protein is 38 kDa, and Catalog#11852-1-AP specially recognises the 38 kDa protein. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2422 Product name: Recombinant human GIMAP4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-329 aa of BC020657 Sequence: MAAQYGSMSFNPSTPGASYGPGRQEPRNSQLRIVLVGKTGAGKSATGNSILGRKVFHSGTAAKSITKKCEKRSSSWKETELVVVDTPGIFDTEVPNAETSKEIIRCILLTSPGPHALLLVVPLGRYTEEEHKATEKILKMFGERARSFMILIFTRKDDLGDTNLHDYLREAPEDIQDLMDIFGDRYCALNNKATGAEQEAQRAQLLGLIQRVVRENKEGCYTNRMYQRAEEEIQKQTQAMQELHRVELEREKARIREEYEEKIRKLEDKVEQEKRKKQMEKKLAEQEAHYAVRQQRARTEVESKDGILELIMTALQIASFILLRLFAED Predict reactive species Full Name: GTPase, IMAP family member 4 Calculated Molecular Weight: 329 aa, 38 kDa Observed Molecular Weight: 38 kDa GenBank Accession Number: BC020657 Gene Symbol: GIMAP4 Gene ID (NCBI): 55303 RRID: AB_2279105 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NUV9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.