Proteintech, 11908-1-AP, ATF2 Polyclonal antibody

Size: 20ul / 150ul The ATF2 (11908-1-AP) by Proteintech is a Polyclonal antibody targeting ATF2 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 11908-1-AP targets ATF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, mouse brain tissue, HeLa cells, K-562 cells Positive IP detected in: mouse brain tissue Positive IF/ICC detected in: HeLa cells, PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody can recognize all the isoforms of human ATF2. The molecular weight detected is larger than the theoretical value, which may be related to protein modification. About 65-68 kDa band detected in the articles(PMID: 19135136; PMID: 28628081 ). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2581 Product name: Recombinant human ATF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC026175 Sequence: MKFKLHVNSARQYKDLWNMSDDKPFLCTAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARNDSVIVADQTPTPTRFLKNCEEVGLFNELASPFENEFKKASEDDIKKMPLDLSPLATPIIRSKIEEPSVVETTHQDSPLPHPESTTSDEKEVPLAQTAQPTSAIVRPASLQVPNVLLTSSDSSVIIQQAVPSPTSSTVITQAPSSNRPIV Predict reactive species Full Name: activating transcription factor 2 Calculated Molecular Weight: 209 aa, 23 kDa Observed Molecular Weight: 60 kDa, 64 kDa GenBank Accession Number: BC026175 Gene Symbol: ATF2 Gene ID (NCBI): 1386 RRID: AB_10695892 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P15336 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.