Proteintech, 11914-1-AP, PLRG1 Polyclonal antibody

Size: 20ul / 150ul The PLRG1 (11914-1-AP) by Proteintech is a Polyclonal antibody targeting PLRG1 in WB, IP, IHC, ELISA applications with reactivity to human samples 11914-1-AP targets PLRG1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, HEK-293 cells, BxPC-3 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PLRG1, identifed as a component of the CDC5L complex, is one spliceosomal protein highly conserved across species. It has been shown to be essential for pre-mRNA splicing in yeast and humans. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2527 Product name: Recombinant human PLRG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 203-505 aa of BC020786 Sequence: VEPGNQWFVTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKVIRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVRCQAAEPQIITGSHDTTIRLWDLVAGKTRVTLTNHKKSVRAVVLHPRHYTFASGSPDNIKQWKFPDGSFIQNLSGHNAIINTLTVNSDGVLVSGADNGTMHLWDWRTGYNFQRVHAAVQPGSLDSESGIFACAFDQSESRLLTAEADKTIKVYREDDTATEETHPVSWKPEIIKRKRF Predict reactive species Full Name: pleiotropic regulator 1 (PRL1 homolog, Arabidopsis) Calculated Molecular Weight: 514 aa, 57 kDa Observed Molecular Weight: 55-60 kDa GenBank Accession Number: BC020786 Gene Symbol: PLRG1 Gene ID (NCBI): 5356 RRID: AB_2165608 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43660 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.