Proteintech, 11965-1-AP, EMG1 Polyclonal antibody

Size: 20ul / 150ul The EMG1 (11965-1-AP) by Proteintech is a Polyclonal antibody targeting EMG1 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 11965-1-AP targets EMG1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, PC-3 cells Positive IP detected in: HeLa cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information EMG1, also named as NEP1, is a highly conserved protein initially identified as "Essential for Mitotic Growth" in yeast. EMG1 is required for maturation of the 18S rRNA and biogenesis of the 40S ribosomal subunit. EMG1 is the methyltransferase in the biosynthesis of m1acp3-Psi in eukaryotic 18S rRNAs. It has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity. A mutation in human EMG1 gene causes Bowen-Conradi syndrome (BCS), an autosomal recessive disorder characterized by severely impaired prenatal and postnatal growth, profound psychomotor retardation, and death in early childhood. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2575 Product name: Recombinant human EMG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC055314 Sequence: MAAPSDGFKPRERSGGEQAQDWDALPPKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSDVRELVPSSDPIVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI Predict reactive species Full Name: EMG1 nucleolar protein homolog (S. cerevisiae) Calculated Molecular Weight: 244 aa, 27 kDa Observed Molecular Weight: 27 kDa GenBank Accession Number: BC055314 Gene Symbol: EMG1 Gene ID (NCBI): 10436 RRID: AB_874093 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92979 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.