Proteintech, 11979-1-AP, EXOSC8 Polyclonal antibody

Size: 20ul / 150ul The EXOSC8 (11979-1-AP) by Proteintech is a Polyclonal antibody targeting EXOSC8 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 11979-1-AP targets EXOSC8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, human brain tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: 4T1 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:800-1:3200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information EXOSC8 (exosome component 8), also known as p9 or CIP3, is a component of the exosome multi-enzyme ribonuclease complex, that degrades or processes messenger RNA. It belongs to the RNase PH family and localizes to the nucleolus. EXOSC8 is one of the six RNase-PH domain subunits of the exosome. EXOSC8 is part of the central hexamer channel of the exosome. EXOSC8 mutations could alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2605 Product name: Recombinant human EXOSC8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-276 aa of BC020773 Sequence: MAAGFKTVEPLEYYRRFLKENCRPDGRELGEFRTTTVNIGSISTADGSALVKLGNTTVICGVKAEFAAPSTDAPDKGYVVPNVDLPPLCSSRFRSGPPGEEAQVASQFIADVIENSQIIQKEDLCISPGKLVWVLYCDLICLDYDGNILDACTFALLAALKNVQLPEVTINEETALAEVNLKKKSYLNIRTHPVATSFAVFDDTLLIVDPTGEEEHLATGTLTIVMDEEGKLCCLHKPGGSGLTGAKLQDCMSRAVTRHKEVKKLMDEVIKSMKPK Predict reactive species Full Name: exosome component 8 Calculated Molecular Weight: 276 aa, 30 kDa Observed Molecular Weight: 30-35 kDa GenBank Accession Number: BC020773 Gene Symbol: EXOSC8 Gene ID (NCBI): 11340 RRID: AB_2101981 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96B26 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.