Proteintech, 12028-1-AP, Cadherin-11 Polyclonal antibody

Size: 20ul / 150ul The Cadherin-11 (12028-1-AP) by Proteintech is a Polyclonal antibody targeting Cadherin-11 in WB, ELISA applications with reactivity to human samples 12028-1-AP targets Cadherin-11 in WB, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: PC-3 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Background Information Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-11 (CDH11), also known osteoblast cadherin (OB-cadherin) or OSF-4, was identified initially in a mouse osteoblastic cell line and human osteosarcoma (PMID: 8163513). Cadherin-11 is a single pass transmembrane protein consisting of an extracellular domain which contains five repeat sequences (EC1-EC5), a transmembrane segment and a cytoplasmic domain which links catenins to the actin and microtubule cytoskeleton (PMID: 8163513; 33442417). It has been shown that cadherin-11 plays important roles in the occurrence and development of a lot of diseases, arthritis, cancer, aortic calcification disease, dermatosis (PMID: 33442417). Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2659 Product name: Recombinant human CDH11 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 29-380 aa of BC013609 Sequence: GHLRPSFHGHHEKGKEGQVLQRSKRGWVWNQFFVIEEYTGPDPVLVGRLHSDIDSGDGNIKYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQYTLMAQAVDRDTNRPLEPPSEFIVKVQDINDNPPEFLHETYHANVPERSNVGTSVIQVTASDADDPTYGNSAKLVYSILEGQPYFSVEAQTGIIRTALPNMDREAKEEYHVVIQAKDMGGHMGGLSGTTKVMITLTDVNDNPPKFPQSVYQMSVSEAAVPGEEVGRVKAKDPDIGENGLVTYNIVDGDGMESFEITTDYETQEGVIKLKKPVDFETKRAYSLKVEAANVHIDPKFISNGPFKDTVTVKIAVEDADEP Predict reactive species Full Name: cadherin 11, type 2, OB-cadherin (osteoblast) Calculated Molecular Weight: 796 aa, 88 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC013609 Gene Symbol: CDH11 Gene ID (NCBI): 1009 RRID: AB_3085386 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P55287 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.