Proteintech, 12032-1-AP, KCNIP3 Polyclonal antibody

Size: 20ul / 150ul The KCNIP3 (12032-1-AP) by Proteintech is a Polyclonal antibody targeting KCNIP3 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 12032-1-AP targets KCNIP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, human brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information KCNIP3 also known as Calsenilin, CSEN, DREAM, or KCHIP3, is a protein-coding gene located on human chromosome 2q11.1 . It encodes a small calcium-binding protein belonging to the recoverin branch of the EF-hand superfamily. KCNIP3 is an integral subunit of native Kv4 channel complexes, regulating A-type potassium currents in response to intracellular calcium changes, thereby modulating neuronal excitability. KCNIP3 interacts with presenilin 1 (PSEN1) and presenilin 2 (PSEN2), influencing γ-secretase activity and β-amyloid formation, potentially linking it to Alzheimer's disease pathology. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2665 Product name: Recombinant human KCNIP3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-256 aa of BC012850 Sequence: MQPAKEVTKASDGSLLGDLGHTPLSKKEGIKWQRPRLSRQALMRCCLVKWILSSTAPQGSDSSDSELELSTVRHQPEGLDQLQAQTKFTKKELQSLYRGFKNECPTGLVDEDTFKLIYAQFFPQGDATTYAHFLFNAFDADGNGAIHFEDFVVGLSILLRGTVHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRHTYPILREDAPAEHVERFFEKMDRNQDGVVTIEEFLEACQKDENIMSSMQLFENVI Predict reactive species Full Name: Kv channel interacting protein 3, calsenilin Calculated Molecular Weight: 256 aa, 29 kDa Observed Molecular Weight: 29 kDa, 27 kDa GenBank Accession Number: BC012850 Gene Symbol: KCNIP3 Gene ID (NCBI): 30818 RRID: AB_2234348 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y2W7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.