Proteintech, 12058-1-AP, ODF2 Polyclonal antibody

Size: 20ul / 150ul The ODF2 (12058-1-AP) by Proteintech is a Polyclonal antibody targeting ODF2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12058-1-AP targets ODF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, mouse lung tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2693 Product name: Recombinant human ODF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 464-763 aa of BC010629 Sequence: QQLKASVKNYEGMIDNYKSQVMKTRLEADEVAAQLERCDKENKILKDEMNKEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAIISDLRSRIEHQGDKLEMAREKHQASQKENKQLSLKVDELERKLEATSAQNIEFLQVIAKREEAIHQSQLRLEEKTRECGTLARQLESAIEDARRQVEQTKEHALSKERAAQNKILDLETQLSRTKTELSQLRRSRDDADRRYQSRLQDLKDRLEQSESTNRSMQNYVQFLKSSYANVFGDGPYSTFLTSSPIRSRSPPA Predict reactive species Full Name: outer dense fiber of sperm tails 2 Calculated Molecular Weight: 763 aa, 89 kDa Observed Molecular Weight: 67-100 kDa GenBank Accession Number: BC010629 Gene Symbol: ODF2 Gene ID (NCBI): 4957 RRID: AB_2156630 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5BJF6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.