Proteintech, 12064-1-AP, IL-24 Polyclonal antibody

Size: 20ul / 150ul The IL-24 (12064-1-AP) by Proteintech is a Polyclonal antibody targeting IL-24 in IHC, ELISA applications with reactivity to human samples 12064-1-AP targets IL-24 in IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Recombinant protein Positive IHC detected in: human malignant melanoma tissue, human prostate cancer tissue, human colon cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information IL24, also named as MDA-7, MDA7 or ST16, belongs to the IL-10 family. It has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. The induction of IL24 by oncogenes may support tumor growth at the early stages of cancer. The observed molecular weight of IL24 is reported between 25 and 35 kDa, a value higher than the deduced molecular weight of 23 kDa. This difference is likely due to post-translational modifications, such as glycosylation of the endogenously produced molecule. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2701 Product name: Recombinant human IL-24 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC009681 Sequence: MNFQQRLQSLWTLASRPFCPPLLATASQMQMVVLPCLGFTLLLWSQVSGAQGQEFHFGPCQVKGVVPQKLWEAFWAVKDTMQAQDNITSARLLQQEVLQNVSDAESCYLVHTLLEFYLKTVFKNYHNRTVEVRTLKSFSTLANNFVLIVSQLQPSQENEMFSIRDSAHRRFLLFRRAFKQLDVEAALTKALGEVDILLTWMQKFYKL Predict reactive species Full Name: interleukin 24 Calculated Molecular Weight: 207 aa, 24 kDa GenBank Accession Number: BC009681 Gene Symbol: IL-24 Gene ID (NCBI): 11009 RRID: AB_10640304 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13007 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.