Proteintech, 12111-1-AP, DPF2 Polyclonal antibody

Size: 20ul / 150ul The DPF2 (12111-1-AP) by Proteintech is a Polyclonal antibody targeting DPF2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12111-1-AP targets DPF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, Y79 cells, HepG2 cells, mouse thymus tissue, K-562 cells Positive IP detected in: Jurkat cells Positive IHC detected in: human lung cancer tissue, human testis tissue, human pituitary adenoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information DPF2, also named as BAF45D or UBID4, is a 391 amino acid protein, which belongs to the requiem/DPF family. DPF2 may be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells and might also have a role in the development and maturation of lymphoid cells. DPF2 was recently shown to inhibit the myeloid differentiation of hematopoietic stem/progenitor and acute myelogenous leukemia cells. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2756 Product name: Recombinant human DPF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-391 aa of BC014889 Sequence: MAAVVENVVKLLGEQYYKDAMEQCHNYNARLCAERSVRLPFLDSQTGVAQSNCYIWMEKRHRGPGLASGQLYSYPARRWRKKRRAHPPEDPRLSFPSIKPDTDQTLKKEGLISQDGSSLEALLRTDPLEKRGAPDPRVDDDSLGEFPVTNSRARKRILEPDDFLDDLDDEDYEEDTPKRRGKGKSKGKGVGSARKKLDASILEDRDKPYACDICGKRYKNRPGLSYHYAHSHLAEEEGEDKEDSQPPTPVSQRSEEQKSKKGPDGLALPNNYCDFCLGDSKINKKTGQPEELVSCSDCGRSGHPSCLQFTPVMMAAVKTYRWQCIECKCCNICGTSENDDQLLFCDDCDRGYHMYCLTPSMSEPPEGSWSCHLCLDLLKEKASIYQNQNSS Predict reactive species Full Name: D4, zinc and double PHD fingers family 2 Calculated Molecular Weight: 391 aa, 44 kDa Observed Molecular Weight: 44-50 kDa GenBank Accession Number: BC014889 Gene Symbol: DPF2 Gene ID (NCBI): 5977 RRID: AB_2095574 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92785 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.