Proteintech, 12117-1-AP, MCCC2 Polyclonal antibody

Size: 20ul / 150ul The MCCC2 (12117-1-AP) by Proteintech is a Polyclonal antibody targeting MCCC2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12117-1-AP targets MCCC2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human liver tissue Positive IP detected in: mouse kidney tissue Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information MCCC2(Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial) is also named as MCCB and belongs to the AccD/PCCB family. The putative 563-amino acid polypeptide has a calculated molecular mass of 61 kD and contains an N-terminal mitochondrial targeting sequence(PMID:11181649). It catalyzes carboxylation of 3 methylcrotonyl COA to form 3 methylglutaconyl-COA and is probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits. Defects in MCCC2 are the cause of methylcrotonoyl-CoA carboxylase 2 deficiency (MCC2D). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2767 Product name: Recombinant human MCCC2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-329 aa of BC014897 Sequence: MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFTEFKAFYGDTLVTGIKPLEKHKT Predict reactive species Full Name: methylcrotonoyl-Coenzyme A carboxylase 2 (beta) Calculated Molecular Weight: 563 aa, 61 kDa Observed Molecular Weight: 61 kDa GenBank Accession Number: BC014897 Gene Symbol: MCCC2 Gene ID (NCBI): 64087 RRID: AB_2143810 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9HCC0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.