Proteintech, 12169-1-AP, CHFR Polyclonal antibody

Size: 20ul / 150ul The CHFR (12169-1-AP) by Proteintech is a Polyclonal antibody targeting CHFR in WB, IHC, ELISA applications with reactivity to human, mouse samples 12169-1-AP targets CHFR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, multi-cells/tissue Positive IHC detected in: human endometrial cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information The Checkpoint with forkhead and RING finger domains protein(CHFR) is a checkpoint protein that reportedly initiates a cell cycle delay in response to microtubule stress during prophase in mitosis and it has been hypothesized to be a tumor suppressor with a potential role as a biomarker for chemotherapeutic response to Taxol(PMID:17596595). It belongs to the CHFR family. Endogenous Chfr is ubiquitinated and phosphorylated in an MPF-dependent manner in mitotic Xenopus egg extracts(PMID:18504434). It has 5 isoforms produced by alternative splicing. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2811 Product name: Recombinant human CHFR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 344-652 aa of BC012072 Sequence: NNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN Predict reactive species Full Name: checkpoint with forkhead and ring finger domains Calculated Molecular Weight: 664 aa, 73 kDa Observed Molecular Weight: 70-85 kDa GenBank Accession Number: BC012072 Gene Symbol: CHFR Gene ID (NCBI): 55743 RRID: AB_2080821 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96EP1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.