Proteintech, 12172-1-AP, FKBP10/FKBP65 Polyclonal antibody

Size: 20ul / 150ul The FKBP10/FKBP65 (12172-1-AP) by Proteintech is a Polyclonal antibody targeting FKBP10/FKBP65 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 12172-1-AP targets FKBP10/FKBP65 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, human brain tissue, human placenta tissue, human kidney tissue, fetal human brain tissue, HeLa cells, NIH/3T3 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, chicken Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2814 Product name: Recombinant human FKBP10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 233-582 aa of BC016467 Sequence: PFLAYGEKGYGTVIPPQASLVFHVLLIDVHNPKDAVQLETLELPPGCVRRAGAGDFMRYHYNGSLMDGTLFDSSYSRNHTYNTYIGQGYIIPGMDQGLQGACMGERRRITIPPHLAYGENGTGDKIPGSAVLIFNVHVIDFHNPADVVEIRTLSRPSETCNETTKLGDFVRYHYNCSLLDGTQLFTSHDYGAPQEATLGANKVIEGLDTGLQGMCVGERRQLIVPPHLAHGESGARGVPGSAVLLFEVELVSREDGLPTGYLFVWHKDPPANLFEDMDLNKDGEVPPEEFSTFIKAQVSEGKGRLMPGQDPEKTIGDMFQNQDRNQDGKITVDELKLKSDEDEERVHEEL Predict reactive species Full Name: FK506 binding protein 10, 65 kDa Calculated Molecular Weight: 582 aa, 64 kDa Observed Molecular Weight: 64 kDa GenBank Accession Number: BC016467 Gene Symbol: FKBP10 Gene ID (NCBI): 60681 RRID: AB_2102550 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96AY3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.