Proteintech, 12173-1-AP, NARF Polyclonal antibody

Size: 20ul / 150ul The NARF (12173-1-AP) by Proteintech is a Polyclonal antibody targeting NARF in WB, ELISA applications with reactivity to human, mouse, rat samples 12173-1-AP targets NARF in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human liver tissue, human lung tissue, human placenta tissue, mouse testis tissue Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Background Information Prelamin A, is processed in the nucleus to lamin A by removal of its final 18 amino acids, including the cysteine residue in its C-terminal CAAX box, which is farnesylated. Nuclear prelamin A recognition factor (NARF) binds the farnesylated prelamin A C-terminal domain(preAct)(PMID:10514485). NARF, at 52 kDa, also named as IOP2, is a small enough protein to freely diffuse through the nuclear pore complex and may be retained within the nucleus by binding nuclear proteins and it has all of the information necessary to be imported into the nucleus(PMID:10514485). It has 3 isoforms produced by alternative splicing with the molecular mass of 51 kDa, 56 kDa and 46 kDa,respectively. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2816 Product name: Recombinant human NARF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-408 aa of BC016440 Sequence: MKCEHCTRKECSKKTKTDDQENVSADAPSPAQENGEKCDTSKHKVLVVSVCPQSLPYFAAKFNLSVTDASRRLCGFLKSLGVHYVFDTTIAADFSILESQKEFVRRYRQHSEEERTLPMLTSACPGWVRYAERVLGRPITAHLCTAKSPQQVMGSLVKDYFARQQNLSPEKIFHVIVAPCYDKKLEALQESLPPALHGSRGADCVLTSGEIAQIMEQGDLSVRDAAVDTLFGDLKEDKVTRHDGASSDGHLAHIFRHAAKELFNEDVEEVTYRALRNKDFQEVTLEKNGEVVLRFAAAYGFRNIQNMILKLKKGKFPFHFVEVLACAGGCLNGRGQAQTPDGHADKALLRQMEGIYADIPVRRPESSAHVQELYQEWLEGINSPKAREVLHTTYQSQERGTHSLDIKW Predict reactive species Full Name: nuclear prelamin A recognition factor Calculated Molecular Weight: 46 kDa, 53 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC016440 Gene Symbol: NARF Gene ID (NCBI): 26502 RRID: AB_2151027 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UHQ1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.