Proteintech, 12185-1-AP, NXF2 Polyclonal antibody

Size: 20ul / 150ul The NXF2 (12185-1-AP) by Proteintech is a Polyclonal antibody targeting NXF2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 12185-1-AP targets NXF2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information NXF2, also named as Nuclear RNA export factor 2 or TAPL2, is a 626 amino acid protein, which contains one TAP-C domain, one RRM domain, one NTF2 domain, and four LRR repeats. NXF2 belongs to the NXF family and is expressed almost exclusively in testis.NXF2 localizes in the nucleus and is involved in the export of mRNA from the nucleus to the cytoplasm. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2841 Product name: Recombinant human NXF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 126-561 aa of BC015020 Sequence: KVTIPYGIKYDKAWLMNSIQSHCSDRFTPVDFHYVRNRACFFVQDASAASALKDVSYKIYDDENQKICIFVNHSTAPYSVKNKLKPGQMEMLKLTMNKRYNVSQQALDLQNLRFDPDLMGRDIDIILNRRNCMAATLKIIERNFPELLSLNLCNNKLYQLDGLSDITEKAPKVKTLNLSKNKLESAWELGKVKGLKLEELWLEGNPLCSTFSDQSAYVSAIRDCFPKLLRLDGRELSAPVIVDIDSSETMKPCKENFTGSETLKHLVLQFLQQYYSIYDSGDRQGLLGAYHDEACFSLAIPFDPKDSAPSSLCKYFEDSRNMKTLKDPYLKGELLRRTKRDIVDSLSALPKTQHDLSSILVDVWCQTERMLCFSVNGVFKEVEGQSQGSVLAFTRTFIATPGSSSSLCIVNDELFVRDASPQETQSAFSIPVSTLS Predict reactive species Full Name: nuclear RNA export factor 2 Calculated Molecular Weight: 626 aa, 72 kDa Observed Molecular Weight: 72 kDa GenBank Accession Number: BC015020 Gene Symbol: NXF2 Gene ID (NCBI): 56001 RRID: AB_2236531 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9GZY0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.