Proteintech, 12205-1-AP, TRIM27 Polyclonal antibody

Size: 20ul / 150ul The TRIM27 (12205-1-AP) by Proteintech is a Polyclonal antibody targeting TRIM27 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 12205-1-AP targets TRIM27 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, mouse testis tissue, Raji cells Positive IP detected in: mouse testis tissue Positive IHC detected in: human lung cancer tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2849 Product name: Recombinant human TRIM27 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 165-513 aa of BC013580 Sequence: EQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP Predict reactive species Full Name: tripartite motif-containing 27 Calculated Molecular Weight: 513 aa, 58 kDa Observed Molecular Weight: 58 kDa, 41 kDa GenBank Accession Number: BC013580 Gene Symbol: TRIM27 Gene ID (NCBI): 5987 RRID: AB_2256660 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P14373 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.