Proteintech, 12215-1-AP, PDK3 Polyclonal antibody

Size: 20ul / 150ul The PDK3 (12215-1-AP) by Proteintech is a Polyclonal antibody targeting PDK3 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 12215-1-AP targets PDK3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse heart tissue, mouse kidney tissue Positive IP detected in: mouse heart tissue Positive IHC detected in: human heart tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information PDK3([Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial) is also named as PDHK3 and belongs to the PDK/BCKDK protein kinase family. It inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 and 48 kDa. This protein can exsit as a dimer with the molecular weight of 90 kDa(PMID:11978179). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2879 Product name: Recombinant human PDK3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 129-369 aa of BC015948 Sequence: GVIEYKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERL Predict reactive species Full Name: pyruvate dehydrogenase kinase, isozyme 3 Calculated Molecular Weight: 406 aa, 47 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC015948 Gene Symbol: PDK3 Gene ID (NCBI): 5165 RRID: AB_2877836 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15120 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.