Proteintech, 12234-1-AP, RAB38 Polyclonal antibody

Size: 20ul / 150ul The RAB38 (12234-1-AP) by Proteintech is a Polyclonal antibody targeting RAB38 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 12234-1-AP targets RAB38 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A375 cells, NIH/3T3 cells, HaCaT cells, MCF-10A cells Positive IHC detected in: human gliomas tissue, human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RAB38 (also known as rrGTPbp or NY-MEL-1) is a member of the Rab small GTPase family, which regulates intracellular vesicle trafficking and membrane transport. RAB38 is specifically expressed in lysosome-related organelle (LRO)-containing tissues and cells, such as melanocytes and platelets, where it interacts with its paralogue RAB32 to regulate melanosome biogenesis and transport melanogenic enzymes (e.g., tyrosinase, TRP1, and TRP2) from early/recycling endosomes to maturing melanosomes. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2874 Product name: Recombinant human RAB38 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC015808 Sequence: MQAPHKEHLYKLLVIGDLGVGKTSIIKRYVHQNFSSHYRATIGVDFALKVLHWDPETVVRLQLWDIAGQERFGNMTRVYYREAMGAFIVFDVTRPATFEAVAKWKNDLDSKLSLPNGKPVSVVLLANKCDQGKDVLMNNGLKMDQFCKEHGFVGWFETSAKENINIDEASRCLVKHILANECDLMESIEPDVVKPHLTSTKVASCSGCAKS Predict reactive species Full Name: RAB38, member RAS oncogene family Calculated Molecular Weight: 211 aa, 24 kDa Observed Molecular Weight: 24 kDa GenBank Accession Number: BC015808 Gene Symbol: RAB38 Gene ID (NCBI): 23682 RRID: AB_2877839 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P57729 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.