Proteintech, 12267-1-AP, PI16 Polyclonal antibody

Size: 20ul / 150ul The PI16 (12267-1-AP) by Proteintech is a Polyclonal antibody targeting PI16 in WB, IHC, ELISA applications with reactivity to human, mouse samples 12267-1-AP targets PI16 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: SKOV-3 cells, A2780 cells, COLO 320 cells, A549 cells, HeLa cells Positive IHC detected in: human liver cancer tissue, human pancreas cancer tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Peptidase inhibitor 16 (PI16) is localized to chromosome 6p21.2 in human, which is also called as prostate secretory protein 94-binding protein (PSPBP). It is a member of the cysteine-rich secretory proteins, antigen 5, and pathogenesis-related proteins (CAP) superfamily (PMID: 32779397). PI16 is a cysteine-rich protein that is widely expressed in human tissuest (PMID: 18824526). PI16 is involved in extracellular matrix regulation, acts as a tumour suppressor gene and is a new independent prognostic marker of prostate cancer (PMID: 37525118, PMID: 17062675). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2925 Product name: Recombinant human PI16 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC022399 Sequence: MHGSCSFLMLLLPLLLLLVATTGPVGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLCEPIGSPEDAQDLPYLVTEAPSFRATEASDSRKMGTPSSLATGIPAFLVTEVSGSLATKALPAVETQAPTSLATKDPPSMATEAPPCVTTEVPSILAAHSLPSL Predict reactive species Full Name: peptidase inhibitor 16 Calculated Molecular Weight: 463 aa, 49 kDa Observed Molecular Weight: 49 kDa GenBank Accession Number: BC022399 Gene Symbol: PI16 Gene ID (NCBI): 221476 RRID: AB_10697651 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6UXB8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.