Proteintech, 12271-1-AP, UCK1 Polyclonal antibody

Size: 20ul / 150ul The UCK1 (12271-1-AP) by Proteintech is a Polyclonal antibody targeting UCK1 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12271-1-AP targets UCK1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, MCF-7 cells Positive IP detected in: MCF-7 cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP). The deduced 277-amino acid protein has a calculated molecular mass of 31 kDa. UCK1 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2912 Product name: Recombinant human UCK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC015547 Sequence: MASAGGEDCESPAPEADRPHQRPFLIGVSGGTASGKSTVCEKIMELLGQNEVEQRQRKVVILSQDRFYKVLTAEQKAKALKGQYNFDHPDAFDNDLMHRTLKNIVEGKTVEVPTYDFVTHSRLPETTVVYPADVVLFEGILVFYSQEIRDMFHLRLFVDTDSDVRLSRRDKEVCRCDHPTRSGQYGCHQPDRAAHPGHSEW Predict reactive species Full Name: uridine-cytidine kinase 1 Calculated Molecular Weight: 277 aa, 31 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC015547 Gene Symbol: UCK1 Gene ID (NCBI): 83549 RRID: AB_11182173 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9HA47 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.