Proteintech, 12273-1-AP, SSSCA1 Polyclonal antibody

Size: 20ul / 150ul The SSSCA1 (12273-1-AP) by Proteintech is a Polyclonal antibody targeting SSSCA1 in WB, IHC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 12273-1-AP targets SSSCA1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, NIH/3T3 cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about23- 27 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2916 Product name: Recombinant human SSSCA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-199 aa of BC014791 Sequence: MALNGAEVDDFSWEPPTEAETKVLQARRERQDRISRLMGDYLLRGYRMLGETCADCGTILLQDKQRKIYCVACQELDSDVDKDNPALNAQAALSQAREHQLASASELPLGSRPAPQPPVPRPEHCEGAAAGLKAAQGPPAPAVPPNTDVMACTQTALLQKLTWASAELGSSTSLETSIQLCGLIRACAEALRSLQQLQH Predict reactive species Full Name: Sjogren syndrome/scleroderma autoantigen 1 Calculated Molecular Weight: 199 aa, 21 kDa Observed Molecular Weight: 27 kDa GenBank Accession Number: BC014791 Gene Symbol: SSSCA1 Gene ID (NCBI): 10534 RRID: AB_2195902 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60232 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.