Proteintech, 12308-1-AP, IL-27RA Polyclonal antibody

Size: 20ul / 150ul The IL-27RA (12308-1-AP) by Proteintech is a Polyclonal antibody targeting IL-27RA in WB, IF/ICC, ELISA applications with reactivity to human samples 12308-1-AP targets IL-27RA in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HL-60 cells, K-562 cells, TF-1 cells Positive IF/ICC detected in: HL-60 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information IL-27RA, also known as T-cell cytokine receptor WSX-1 or TCCR, is a unique receptor subunit for the cytokine IL-27. It is expressed in a variety of cells including activated T cells, NK cells, mast cells, endothelial cells, activated B cells, monocytes, Langerhans cells, and activated dendritic cells (PMID: 18378322). The interaction with IL-27 leads to the phosphorylation of Jak and STAT proteins, which are crucial for the regulation of immune responses (PMID: 28536468). IL-27RA plays a significant role in immune cell activation and differentiation, particularly in the context of Th1 and Th17 cells (PMID: 38566992). Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2956 Product name: Recombinant human IL-27RA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 33-515 aa of BC028003 Sequence: QGSAGPLQCYGVGPLGDLNCSWEPLGDLGAPSELHLQSQKYRSNKTQTVAVAAGRSWVAIPREQLTMSDKLLVWGTKAGQPLWPPVFVNLETQMKPNAPRLGPDVDFSEDDPLEATVHWAPPTWPSHKVLICQFHYRRCQEAAWTLLEPELKTIPLTPVEIQDLELATGYKVYGRCRMEKEEDLWGEWSPILSFQTPPSAPKDVWVSGNLCGTPGGEEPLLLWKAPGPCVQVSYKVWFWVGGRELSPEGITCCCSLIPSGAEWARVSAVNATSWEPLTNLSLVCLDSASAPRSVAVSSIAGSTELLVTWQPGPGEPLEHVVDWARDGDPLEKLNWVRLPPGNLSALLPGNFTVGVPYRITVTAVSASGLASASSVWGFREELAPLVGPTLWRLQDAPPGTPAIAWGEVPRHQLRGHLTHYTLCAQSGTSPSVCMNVSGNTQSVTLPDLPWGPCELWVTASTIAGQGPPGPILRLHLPDNTLRW Predict reactive species Full Name: interleukin 27 receptor, alpha Calculated Molecular Weight: 636 aa, 69 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC028003 Gene Symbol: IL27RA Gene ID (NCBI): 9466 RRID: AB_3669136 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6UWB1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.