Proteintech, 12313-1-AP, KIN17 Polyclonal antibody

Size: 20ul / 150ul The KIN17 (12313-1-AP) by Proteintech is a Polyclonal antibody targeting KIN17 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 12313-1-AP targets KIN17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, human heart tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information KIN17 (also known as KIN) is an evolutionarily conserved zinc finger nuclear protein. It consists of three motifs, a zinc finger, a nuclear localization signal and a Kyprides-Onzonis-Woese motif that mediates transcription elongation. KIN17 is ubiquitously expressed in human tissues and participates in DNA replication, DNA repair and cell cycle progression. Emerging evidence has indicated the importance of KIN17 in the pathogenesis and progression of tumors (PMID: 28454391). KIN17 has a calculated molecular wight of 45 kDa, this antibody is raised against human KIN17 and detects a band of about 40 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2963 Product name: Recombinant human KIN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-364 aa of BC017309 Sequence: MGKSDFLTPKAIANRIKSKGLQKLRWYCQMCQKQCRDENGFKCHCMSESHQRQLLLASENPQQFMDYFSEEFRNDFLELLRRRFGTKRVHNNIVYNEYISHREHIHMNATQWETLTDFTKWLGREGLCKVDETPKGWYIQYIDRDPETIRRQLELEKKKKQDLDDEEKTAKFIEEQVRRGLEGKEQEVPTFTELSRENDEEKVTFNLSKGACSSSGATSSKSSTLGPSALKTIGSSASVKRKESSQSSTQSKEKKKKKSALDEIMEIEEEKKRTARTDYWLQPEIIVKIITKKLGEKYHKKKAIVKEVIDKYTAVVKMIDSGDKLKLDQTHLETVIPAPGKRILVLNGGYRGNEGTLESINEKT Predict reactive species Full Name: KIN, antigenic determinant of recA protein homolog (mouse) Calculated Molecular Weight: 393 aa, 45 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC017309 Gene Symbol: KIN17 Gene ID (NCBI): 22944 RRID: AB_2130343 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60870 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.