Proteintech, 12339-1-AP, SDPR Polyclonal antibody

Size: 20ul / 150ul The SDPR (12339-1-AP) by Proteintech is a Polyclonal antibody targeting SDPR in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12339-1-AP targets SDPR in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, mouse brain tissue, mouse liver tissue, COLO 320 cells, mouse placenta tissue, rat brain tissue, mouse heart tissue Positive IP detected in: mouse heart tissue Positive IHC detected in: human renal cell carcinoma tissue, human breast hyperplasia tissue, human heart tissue, human kidney tissue, human normal colon, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Serum deprivation-response protein(SDRP), a substrate of protein kinase C, is a member of a group of proteins that bind to phosphatidylserine in a clacium-dependent manner. SDPR may participate in the generation or stabilization of membrane curvature. SDPR is highly expressed in heart and lung, and low in brain,kidney, liver, pancreas, placenta and skeletal muscle. This is a rabbit polyclonal anti-SDPR antibody raised against C-terminus of human SDPR. SDRP of molecular masses 115 kDa and 100 kDa were found in neutrophils, monocytes, lymphocytes, platelets and erythrocytes. In platelets, however, the principal SDRP is a cytosolic protein of molecular mass 68 kDa. (PMID: 2390065 ) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2990 Product name: Recombinant human SDPR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 73-425 aa of BC016475 Sequence: QENQHKMEQRQISLEGSVKGIQNDLTKLSKYQASTSNTVSKLLEKSRKVSAHTRAVKERMDRQCAQVKRLENNHAQLLRRNHFKVLIFQEENEIPASVFVKQPVSGAVEGKEELPDENKSLEETLHTVDLSSDDDLPHDEEALEDSAEEKVEESRAEKIKRSSLKKVDSLKKAFSRQNIEKKMNKLGTKIVSVERREKIKKSLTSNHQKISSGKSSPFKVSPLTFGRKKVREGESHAENETKSEDLPSSEQMPNDQEEESFAEGHSEASLASALVEGEIAEEAAEKATSRGSNSGMDSNIDLTIVEDEEEESVALEQAQKVRYEGSYALTSEEAERSDGDPVQPAVLQVHQTS Predict reactive species Full Name: serum deprivation response (phosphatidylserine binding protein) Calculated Molecular Weight: 425 aa, 47 kDa Observed Molecular Weight: 68-70 kDa GenBank Accession Number: BC016475 Gene Symbol: SDPR Gene ID (NCBI): 8436 RRID: AB_2183305 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95810 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.