Proteintech, 12372-1-AP, IL-33 Polyclonal antibody

Size: 20ul / 150ul The IL-33 (12372-1-AP) by Proteintech is a Polyclonal antibody targeting IL-33 in WB, ELISA applications with reactivity to human samples 12372-1-AP targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HUVEC cells, Recombinant protein, PMA, LPS and Brefeldin A treated THP-1 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2957 Product name: Recombinant human IL-33 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-270 aa of BC047085 Sequence: MKPKMKYSTNKISTAKWKNTASKALCFKLGKSQQKAKEVCPMYFMKLRSGLMIKKEACYFRRETTKRPSLKTGRKHKRHLVLAACQQQSTVECFAFGISGVQKYTRALHDSSITGISPITEYLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET Predict reactive species Full Name: interleukin 33 Calculated Molecular Weight: 30 kDa Observed Molecular Weight: 31 kDa GenBank Accession Number: BC047085 Gene Symbol: IL-33 Gene ID (NCBI): 90865 RRID: AB_2877852 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95760 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.