Proteintech, 12395-1-AP, MAT1A/MAT2A Polyclonal antibody

Size: 20ul / 150ul The MAT1A/MAT2A (12395-1-AP) by Proteintech is a Polyclonal antibody targeting MAT1A/MAT2A in WB, IHC, ELISA applications with reactivity to human, mouse, rat, arabidopsis samples 12395-1-AP targets MAT1A/MAT2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, arabidopsis samples. Tested Applications Positive WB detected in: mouse liver tissue, arabidopsis whole plant tissue, HCT 116 cells, HeLa cells, HepG2 cells, rat liver tissue Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A. This antibody was raised against the full length of human MAT1A and can recognize both of MAT1A and MAT2A. Western blot analysis using this antibody detected a single band around 44-50 kDa in liver. Specification Tested Reactivity: human, mouse, rat, arabidopsis Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3076 Product name: Recombinant human MAT1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-395 aa of BC018359 Sequence: MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF Predict reactive species Full Name: methionine adenosyltransferase I, alpha Calculated Molecular Weight: 395 aa, 44 kDa Observed Molecular Weight: 47 kDa, 49 kDa GenBank Accession Number: BC018359 Gene Symbol: MAT1A Gene ID (NCBI): 4143 RRID: AB_2266186 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q00266 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.