Proteintech, 12400-1-AP, MAGEB1 Polyclonal antibody

Size: 20ul / 150ul The MAGEB1 (12400-1-AP) by Proteintech is a Polyclonal antibody targeting MAGEB1 in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 12400-1-AP targets MAGEB1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, A375 cells Positive IP detected in: HepG2 cells Positive IF/ICC detected in: HepG2 cells, A375 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information MAGEB1, also named as MAGEL1, MAGEXP, DAM10 and CT3.1, is a member of the MAGEB gene family. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. The MW of MAGEB1 is about 40-45kd. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3094 Product name: Recombinant human MAGEB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-347 aa of BC013772 Sequence: MPRGQKSKLRAREKRRKAREETHYLKVAHATAAEKEECPSSSPVLGDTPTSSPAAGIPQKPQGAPPTTTAAAAVSCTESDEGAKCQGEENASFSQATTSTESSVKDPVAWEAGMLMHFILRKYKMREPIMKADMLKVVDEKYKEYFTEILNGASRRLELVFGLDLKEDNPSGHTYTLVSKLNLTNDGNLSNDWDFPRNGLLMPLLGVIFLKGNSATEEEIWKFMNVLGAYDGEEHLIYGEPRKFITQDLVQEKYLKYEQVPNSDPPRYQFLWGPRAYAETTKMKVLEFLAKMNGATPRDFPSHYEEALRDEEERAQVRSSVRARRRTTATTFRARSRAPFSRSSHPM Predict reactive species Full Name: melanoma antigen family B, 1 Calculated Molecular Weight: 347 aa, 39 kDa Observed Molecular Weight: 43-45 kDa GenBank Accession Number: BC013772 Gene Symbol: MAGEB1 Gene ID (NCBI): 4112 RRID: AB_2877854 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P43366 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.