Proteintech, 12447-1-AP, TBX6 Polyclonal antibody

Size: 20ul / 150ul The TBX6 (12447-1-AP) by Proteintech is a Polyclonal antibody targeting TBX6 in WB, IP, ELISA applications with reactivity to human, mouse samples 12447-1-AP targets TBX6 in WB, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, A375 cells, A549 cells, human lung tissue, human placenta tissue, mouse lung tissue, Raji cells Positive IP detected in: human placenta tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information TBX6, also named as T-box transcription factor TBX6, is a 436 amino acid protein, which localizes in the nucleus and forms a dimeric complex with DNA. T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. The calculated molecular weight of TBX6 is 47 kDa, but the modified TBX6 is about 58 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3050 Product name: Recombinant human TBX6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 87-436 aa of BC026031 Sequence: SLPGVSLSLENRELWKEFSSVGTEMIITKAGRRMFPACRVSVTGLDPEARYLFLLDVIPVDGARYRWQGRRWEPSGKAEPRLPDRVYIHPDSPATGAHWMRQPVSFHRVKLTNSTLDPHGHLILHSMHKYQPRIHLVRAAQLCSQHWGGMASFRFPETTFISVTAYQNPQITQLKIAANPFAKGFRENGRNCKRERDARVKRKLRGPEPAATEAYGSGDTPGGPCDSTLGGDIRESDPEQAPAPGEATAAPAPLCGGPSAEAYLLHPAAFHGAPSHLPTRSPSFPEAPDSGRSAPYSAAFLELPHGSGGSGYPAAPPAVPFAPHFLQGGPFPLPYTAPGGYLDVGSKPMY Predict reactive species Full Name: T-box 6 Calculated Molecular Weight: 436 aa, 47 kDa Observed Molecular Weight: 58 kDa GenBank Accession Number: BC026031 Gene Symbol: TBX6 Gene ID (NCBI): 6911 RRID: AB_2200836 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95947 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.