Proteintech, 12460-1-AP, PAK4/6/7 Polyclonal antibody

Size: 20ul / 150ul The PAK4/6/7 (12460-1-AP) by Proteintech is a Polyclonal antibody targeting PAK4/6/7 in IP, IHC, ELISA applications with reactivity to human, mouse samples 12460-1-AP targets PAK4/6/7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. The antibody can recognize PAK4/6/7. PAK4/6/7 belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It acts on a variety of targets. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3127 Product name: Recombinant human PAK7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 420-719 aa of BC024179 Sequence: SDQQPSRVSHEQFRAALQLVVSPGDPREYLANFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRRELLFNEVVIMRDYHHDNVVDMYSSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATVCLSVLRALSYLHNQGVIHRDIKSDSILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSLPPRVKDLHKVSSVLRGFLDLMLVREPSQRATAQELLGHPFLKLAGPPSCIVPLMRQYRHH Predict reactive species Full Name: p21 protein (Cdc42/Rac)-activated kinase 7 Calculated Molecular Weight: 719 aa, 81 kDa Observed Molecular Weight: 80 kDa GenBank Accession Number: BC024179 Gene Symbol: PAK7 Gene ID (NCBI): 57144 RRID: AB_2877858 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P286 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.