Proteintech, 12484-1-AP, TRAPPC2 Polyclonal antibody

Size: 20ul / 150ul The TRAPPC2 (12484-1-AP) by Proteintech is a Polyclonal antibody targeting TRAPPC2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 12484-1-AP targets TRAPPC2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, human brain tissue, human liver tissue, human lung tissue, mouse thymus tissue, PC-3 cells, rat brain tissue Positive IP detected in: PC-3 cells Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TRAPPC2 is one component of the TRAPP multisubunit tethering complex involved in intracellular vesicle trafficking [PMID:21525244]. It acts as an adaptor for the TRAPP complex in mammalian cells, mediating interactions with both TRAPPC9 and TRAPPC8. It prevents transcriptional repression and induction of cell death by ENO1. Also it plays a role in vesicular transport from endoplasmic reticulum to Golgi. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3201 Product name: Recombinant human TRAPPC2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC016915 Sequence: MSGSFYFVIVGHHDNPVFEMEFLPAGKAESKDDHRHLNQFIAHAALDLVDENMWLSNNMYLKTVDKFNEWFVSAFVTAGHMRFIMLHDIRQEDGIKNFFTDVYDLYIKFSMNPFYEPNSPIRSSAFDRKVQFLGKKHLLS Predict reactive species Full Name: trafficking protein particle complex 2 Calculated Molecular Weight: 140 aa, 16 kDa Observed Molecular Weight: 16 kDa GenBank Accession Number: BC016915 Gene Symbol: TRAPPC2 Gene ID (NCBI): 6399 RRID: AB_2208136 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P0DI82 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.