Proteintech, 12488-1-AP, TBL2 Polyclonal antibody

Size: 20ul / 150ul The TBL2 (12488-1-AP) by Proteintech is a Polyclonal antibody targeting TBL2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 12488-1-AP targets TBL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: COLO 320 cells, HeLa cells, human placenta tissue, Jurkat cells, mouse pancreas tissue, mouse placenta tissue, Caco-2 cells Positive IP detected in: COLO 320 cells Positive IHC detected in: human pancreas cancer tissue, human heart tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3200 Product name: Recombinant human TBL2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 100-446 aa of BC012938 Sequence: SNGKYLATCADDRTIRIWSTKDFLQREHRSMRANVELDHATLVRFSPDCRAFIVWLANGDTLRVFKMTKREDGGYTFTATPEDFPKKHKAPVIDIGIANTGKFIMTASSDTTVLIWSLKGQVLSTINTNQMNNTHAAVSPCGRFVASCGFTPDVKVWEVCFGKKGEFQEVVRAFELKGHSAAVHSFAFSNDSRRMASVSKDGTWKLWDTDVEYKKKQDPYLLKTGRFEEAAGAAPCRLALSPNAQVLALASGSSIHLYNTRRGEKEECFERVHGECIANLSFDITGRFLASCGDRAVRLFHNTPGHRAMVEEMQGHLKRASNESTRQRLQQQLTQAQVTLGGPGAED Predict reactive species Full Name: transducin (beta)-like 2 Calculated Molecular Weight: 446 aa, 50 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC012938 Gene Symbol: TBL2 Gene ID (NCBI): 26608 RRID: AB_2199923 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y4P3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.