Proteintech, 12550-1-AP, PER3 Polyclonal antibody

Size: 20ul / 150ul The PER3 (12550-1-AP) by Proteintech is a Polyclonal antibody targeting PER3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 12550-1-AP targets PER3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: COLO 320 cells, HeLa cells, Calu-1 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The suprachiasmatic nuclei (SCN) of the anterior hypothalamus function as a circadian clock in the mammalian brain, generating circadian rhythms in physiology and behavior. The period homolog genesPer3 is an important component of the circadian clock system. PER3 plays a role in cell proliferation, apoptosis,DNA damage response, cell cycle control, and treatment response of chemotherapy agents in cancers. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3400 Product name: Recombinant human PER3 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 17-378 aa of BC026102 Sequence: EALGEESGERWSPEFHLQRKLADSSHSEQQDRNRVSEELIMVVQEMKKYFPSERRNKPSTLDALNYALRCVHSVQANSEFFQILSQNGAPQADVSMYSLEELATIASEHTSKNTDTFVAVFSFLSGRLVHISEQAALILNRKKDVLASSHFVDLLAPQDMRVFYAHTARAQLPFWNNWTQRAARYECAPVKPFFCRIRGGEDRKQEKCHSPFRIIPYLIHVHHPAQPELESEPCCLTVVEKIHSGYEAPRIPVNKRIFTTTHTPGCVFLEVDEKAVPLLGYLPQDLIGTSILSYLHPEDRSLMVAIHQKVLKYAGHPPFEHSPIRFCTQNGDYIILDSSWSSFVNPWSRKISFIIGRHKVRT Predict reactive species Full Name: period homolog 3 (Drosophila) Calculated Molecular Weight: 1201 aa, 132 kDa Observed Molecular Weight: 132 kDa GenBank Accession Number: BC026102 Gene Symbol: PER3 Gene ID (NCBI): 8863 RRID: AB_2161814 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P56645 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.