Proteintech, 12624-1-AP, RBBP8 Polyclonal antibody

Size: 20ul / 150ul The RBBP8 (12624-1-AP) by Proteintech is a Polyclonal antibody targeting RBBP8 in WB, ELISA applications with reactivity to human, mouse samples 12624-1-AP targets RBBP8 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Raji cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information RBBP8 is an endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. It functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. It interacted with BRCA1 to form a complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Also it promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3314 Product name: Recombinant human RBBP8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 604-902 aa of BC030590 Sequence: KSAGSHEPIKIQTRSDHGGCELASVLQLNPCRTGKIKSLQNNQDVSFENIQWSIDPGADLSQYKMDVTVIDTKDGSQSKLGGETVDMDCTLVSETVLLKMKKQEQKGEKSSMLFYIDEERKMNDSLEDMFDRTTHEEYESCLADSFSQAADEEEELSTATKKLHTHGDKQDKVKQKAFVEPYFKGDERETSLQNFPHIEVVRKKEERRKLLGHTCKECEIYYADMPAEEREKKLASCSRHRFRYIPPNTPENFWEVGFPSTQTCMERGYIKEDLDPCPRPKRRQPYNAIFSPKGKEQKT Predict reactive species Full Name: retinoblastoma binding protein 8 Calculated Molecular Weight: 103 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC030590 Gene Symbol: RBBP8 Gene ID (NCBI): 5932 RRID: AB_2175250 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99708 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.