Proteintech, 12641-1-AP, SHC4 Polyclonal antibody

Size: 20ul / 150ul The SHC4 (12641-1-AP) by Proteintech is a Polyclonal antibody targeting SHC4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 12641-1-AP targets SHC4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SH-SY5Y cells, human brain tissue, HepG2 cells, human skeletal muscle tissue, mouse brain tissue, A375 cells, rat brain tissue Positive IHC detected in: rat brain tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3336 Product name: Recombinant human SHC4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 324-630 aa of BC033907 Sequence: NGMAQDVISTIGQAFELRFKQYLKNPSLNTSCESEEVHIDSHAEEREDHEYYNEIPGKQPPVGGVSDMRIKVQATEQMAYCPIQCEKLCYLPGNSKCSSVYENCLEQSRAIGNVHPRGVQSQRGTSLLKHTCRVDLFDDPCYINTQALQSTPGSAGNQRSAQPLGSPWHCGKAPETVQPGATAQPASSHSLPHIKQQLWSEECYHGKLSRKAAESLLVKDGDFLVRESATSPGQYVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIRYHMDNSLPIISSGSEVSLKQPVRKDNNPALLHSNK Predict reactive species Full Name: SHC (Src homology 2 domain containing) family, member 4 Calculated Molecular Weight: 630 aa, 69 kDa Observed Molecular Weight: 69 kDa GenBank Accession Number: BC033907 Gene Symbol: SHC4 Gene ID (NCBI): 399694 RRID: AB_10643243 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6S5L8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.