Proteintech, 12655-1-AP, IL-5RA/CD125 Polyclonal antibody

Size: 20ul / 150ul The IL-5RA/CD125 (12655-1-AP) by Proteintech is a Polyclonal antibody targeting IL-5RA/CD125 in WB, ELISA applications with reactivity to human samples 12655-1-AP targets IL-5RA/CD125 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human peripheral blood platelets, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information Interleukin-5 receptor subunit alpha (IL-5RA), also known as CD125, is a type I transmembrane protein that belongs to the type I cytokine receptor family. IL-5RA is a critical component of the high-affinity IL-5 receptor complex, IL-5R, which consists of the alpha chain (IL-5RA) that specifically binds to IL-5 and the common beta chain (CD131) shared with receptors for IL-3 and GM-CSF (PMID: 1833065; 7872157). IL-5/IL-5RA signaling axis is crucial for the differentiation, proliferation, recruitment, and activation of eosinophils, and is also involved in pathological eosinophilia (PMID: 34638583). Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3358 Product name: Recombinant human IL-5RA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 50-335 aa of BC027599 Sequence: WKPNPDQEQRNVNLEYQVKINAPKEDDYETRITESKCVTILHKGFSASVRTILQNDHSLLASSWASAELHAPPGSPGTSIVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGTDAPEDTQYFLYYRYGSWTEECQEYSKDTLGRNIACWFPRTFILSKGRDWLAVLVDGSSKHSAIRPFDQLFALHAIDQINPPLNVTAEIEGTRLSIQWEKPVSAFPIHCFDYEVKIHNTRNGYLQIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCREAGLWSEWSQPIYVGFSR Predict reactive species Full Name: interleukin 5 receptor, alpha Calculated Molecular Weight: 48 kDa, 38 kDa Observed Molecular Weight: 70-75 kDa, 43 kDa GenBank Accession Number: BC027599 Gene Symbol: IL-5RA/CD125 Gene ID (NCBI): 3568 RRID: AB_2127342 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q01344 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.