Proteintech, 12658-1-AP, P4HA1 Polyclonal antibody

Size: 20ul / 150ul The P4HA1 (12658-1-AP) by Proteintech is a Polyclonal antibody targeting P4HA1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 12658-1-AP targets P4HA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, NIH/3T3 cells, human heart tissue, rat skin tissue, HT-1080 cells, BGC-823 cells, PC-3 cells Positive IP detected in: A431 cells Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information P4HA1 (Prolyl 4-hydroxylase subunit alpha-1) is also known as P4HA and belongs to the P4HA family, which plays a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exist as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has some isoforms with the MW 61-63 kDa and 57-59 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3292 Product name: Recombinant human P4HA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 236-534 aa of BC034998 Sequence: PEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE Predict reactive species Full Name: prolyl 4-hydroxylase, alpha polypeptide I Calculated Molecular Weight: 534 aa, 61 kDa Observed Molecular Weight: 61 kDa GenBank Accession Number: BC034998 Gene Symbol: P4HA1 Gene ID (NCBI): 5033 RRID: AB_2283162 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P13674 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.