Proteintech, 12688-1-AP, BHLHE41 Polyclonal antibody

Size: 20ul / 150ul The BHLHE41 (12688-1-AP) by Proteintech is a Polyclonal antibody targeting BHLHE41 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 12688-1-AP targets BHLHE41 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, rat testis tissue Positive IHC detected in: human ovary tumor tissue, mouse brain tissue, rat heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information BHLHE41 (basic helix-loop-helix family member e41), also called SHARP1 and DEC2, is a member of the BHLH family proteins that are involved in differentiation, circadian rhythms and the response to hypoxia. In esophageal, osteosarcomas, prostate, breast, and endometrial cancer cells, BHLHE41 functions as an anti-apoptotic factor and promotes cell proliferation and invasiveness. It suppresses cell proliferation and metastasis, and also facilitates pancreatic as well as gastric cancer cell apoptosis. BHLHE41 has an important role in the proliferation of glioblastoma and couldserve as a novel candidate for targeted therapy of glioblastoma (PMID: 31616182). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3381 Product name: Recombinant human BHLHE41 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC025968 Sequence: MDEGIPHLQERQLLEHRDFIGLDYSSLYMCKPKRSMKRDDTKDTYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHLKALTALTEQQHQKIIALQNGERSLKSPIQSDLDAFHSGFQTCAKEVLQYLSRFESWTPREPRCVQLINHLHAVATQFLPTPQLLTQQVPLSKGTGAPSAAGSAAAPCLERAGQKLEPLAYCVPVIQRTQPSAELAAENDTDTDSGYGGEAEARPDREKGKGAGASRVTIKQEPPGEDSPAPKRMKLDSRGGGSGGGPGGGAVAAAAALLGPDPAAAAALLRPDAALLSSLVAFGGGGGAPFPQPAAAAAPFCLPFC Predict reactive species Full Name: basic helix-loop-helix family, member e41 Calculated Molecular Weight: 482 aa, 51 kDa Observed Molecular Weight: 50-52 kDa GenBank Accession Number: BC025968 Gene Symbol: BHLHE41 Gene ID (NCBI): 79365 RRID: AB_2065361 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9C0J9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.