Proteintech, 12693-1-AP, MNS1 Polyclonal antibody

Size: 20ul / 150ul The MNS1 (12693-1-AP) by Proteintech is a Polyclonal antibody targeting MNS1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12693-1-AP targets MNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: rat testis tissue Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Meiosis-specific nuclear structural 1 (MNS1), is abundantly expressed in post-meiotic spermatids and is required for proper flagellar formation and function. Male mice deficient in MNS1 display decreased sperm production and are sterile because their sperm are immotile. The microtubules and ODFs of the flagella are disrupted, resulting in abnormal, short sperm tails. In addition, MNS1 is also required for motile ciliary functions. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3423 Product name: Recombinant human MNS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 189-495 aa of BC034991 Sequence: LDLEKQLEEQEKKKQEAYEQLLKEKLMIDEIVRKIYEEDQLEKQQKLEKMNAMRRYIEEFQKEQALWRKKKREEMEEENRKIIEFANMQQQREEDRMAKVQENEEKRLQLQNALTQKLEEMLRQREDLEQVRQELYQEEQAEIYKSKLKEEAEKKLRKQKEMKQDFEEQMALKELVLQAAKEEEENFRKTMLAKFAEDDRIELMNAQKQRMKQLEHRRAVEKLIEERRQQFLADKQRGLEEWQLQQRRQGFINAIIEEERLKLLKEHATNLLGYLPKGVFKKEDDIDLLGEEFRKVYQQRSEICEEK Predict reactive species Full Name: meiosis-specific nuclear structural 1 Calculated Molecular Weight: 495 aa, 61 kDa Observed Molecular Weight: 55-60 kDa GenBank Accession Number: BC034991 Gene Symbol: MNS1 Gene ID (NCBI): 55329 RRID: AB_2877875 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NEH6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.