Proteintech, 12735-1-AP, RabGEF1 Polyclonal antibody

Size: 20ul / 150ul The RabGEF1 (12735-1-AP) by Proteintech is a Polyclonal antibody targeting RabGEF1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 12735-1-AP targets RabGEF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-3 cells Positive IHC detected in: human endometrial cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information RabGEF1 (Rabex-5) is a guanine nucleotide exchange factor (GEF) for the endocytic pathway regulator Rab5. RabGEF1 contains a Vps9 domain, an A20-like zinc finger (ZnF) domain, and a coiled coil domain (PMID: 17341663). RabGEF1 is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It is also implicated in tumorigenesis and in the development of certain human cancers (PMID: 25427001). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3442 Product name: Recombinant human RABGEF1 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 136-491 aa of BC015330 Sequence: DRVSKEFIEFLKTFHKTGQEIYKQTKLFLEGMHYKRDLSIEEQSECAQDFYHNVAERMQTRGKVPPERVEKIMDQIEKYIMTRLYKYVFCPETTDDEKKDLAIQKRIRALRWVTPQMLCVPVNEDIPEVSDMVVKAITDIIEMDSKRVPRDKLACITKCSKHIFNAIKITKNEPASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAVAFIEKLDAQSLNLSQEDFDRYMSGQTSPRKQEAESWSPDACLGVKQMYKNLDLLSQLNERQERIMNEAKKLEKDLIDWTDGIAREVQDIVEKYPLEIKPPNQPLAAIDSENVENDKLPPPLQPQVYAG Predict reactive species Full Name: RAB guanine nucleotide exchange factor (GEF) 1 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC015330 Gene Symbol: RABGEF1 Gene ID (NCBI): 27342 RRID: AB_2175788 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UJ41 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.