Proteintech, 12747-1-AP, PPP2R2C Polyclonal antibody

Size: 20ul / 150ul The PPP2R2C (12747-1-AP) by Proteintech is a Polyclonal antibody targeting PPP2R2C in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12747-1-AP targets PPP2R2C in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, mouse testis tissue Positive IHC detected in: human breast cancer tissue, human brain tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Protein phosphatase 2 regulatory subunit B gamma, also known as PPP2R2C or B55-gamma, encodes a gamma isoform of the subunit B55 subfamily, which is a regulatory subunit of Protein phosphatase type 2A (PP2A) (PMID: 24126060). PPP2R2C has been identified as a potential tumor suppressor in several cancers, including prostate and brain cancers (PMID: 23493267, PMID: 24126060). The decreased expression of PPP2R2C is associated with tumor progression, metastasis, and poorer patient outcomes in prostate cancer. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3470 Product name: Recombinant human PPP2R2C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 131-430 aa of BC032954 Sequence: LQVPVLKPMDLMVEVSPRRIFANGHTYHINSISVNSDCETYMSADDLRINLWHLAITDRSFNIVDIKPANMEDLTEVITASEFHPHHCNLFVYSSSKGSLRLCDMRAAALCDKHSKLFEEPEDPSNRSFFSEIISSVSDVKFSHSGRYMLTRDYLTVKVWDLNMEARPIETYQVHDYLRSKLCSLYENDCIFDKFECAWNGSDSVIMTGAYNNFFRMFDRNTKRDVTLEASRESSKPRAVLKPRRVCVGGKRRRDDISVDSLDFTKKILHTAWHPAENIIAIAATNNLYIFQDKVNSDMH Predict reactive species Full Name: protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform Calculated Molecular Weight: 430 aa, 49 kDa Observed Molecular Weight: 49 kDa GenBank Accession Number: BC032954 Gene Symbol: PPP2R2C Gene ID (NCBI): 5522 RRID: AB_2170099 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y2T4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.