Proteintech, 12807-1-AP, BACE1 Polyclonal antibody

Size: 20ul / 150ul The BACE1 (12807-1-AP) by Proteintech is a Polyclonal antibody targeting BACE1 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications with reactivity to human, mouse, rat, zebrafish samples 12807-1-AP targets BACE1 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples. Tested Applications Positive WB detected in: mouse brain tissue, human brain tissue, mouse pancreas tissue, HeLa cells, zebrafish tissue, PC-12 cells, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse brain tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Positive IF-Fro detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800 Background Information beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680). Specification Tested Reactivity: human, mouse, rat, zebrafish Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3559 Product name: Recombinant human BACE1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 157-457 aa of BC036084 Sequence: VRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLM Predict reactive species Full Name: beta-site APP-cleaving enzyme 1 Calculated Molecular Weight: 501 aa, 56 kDa Observed Molecular Weight: 70-75 kDa, 42-56 kDa GenBank Accession Number: BC036084 Gene Symbol: BACE1 Gene ID (NCBI): 23621 RRID: AB_10638760 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P56817 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.