Proteintech, 12816-1-AP, ITPKB Polyclonal antibody

Size: 20ul / 150ul The ITPKB (12816-1-AP) by Proteintech is a Polyclonal antibody targeting ITPKB in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 12816-1-AP targets ITPKB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, K-562 cells, mouse lung tissue, mouse thymus tissue, rat brain tissue Positive IHC detected in: human cervical cancer tissue, human lung tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Inositol 1,4,5-trisphosphate 3-kinase isoform B (IITPKB) is also named as IP3KB and possesses important roles in the development of immune cells. IP3KB can be targeted to multiple cellular compartments, among them nuclear localization and binding in close proximity to the plasma membrane. The B isoform is the only IP3K that is almost ubiquitously expressed in mammalian cells(PMID:21148483).Three isoforms of IP3KB occur in mammals, which are distinguished by different molecular masses, sensitivity to Ca2+/CaM(calmodulin), intracellular distribution and tissue expression (PMID:16173920). ITPKB can be cleaved into many fragments, this antibody can recognize the N-terminal peptide fragment.(PMID:15130091) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3456 Product name: Recombinant human ITPKB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC015009 Sequence: MAVYCYALNSLVIMNSANEMKSGGGPGPSGSETPPPPRRAVLSPGSVFSPGRGASFLFPPAESLSPEEPRSPGGWRSGRRRLNSSSGSGSGSSGSSVSSPSWAGRLRGDRQQVVAAGTLSPPGPEEAKRKLRILQRELQNVQVNQKVGMFEAHIQAQSSAIQAPRSPRLGRAHSPSPCPFRSSSQPPGRVLVQGARSEERRTKSWGEQCPETSGTDSGRKGGPSLCSSQVKKGMPPLPGRAAPTGSEAQGPSAFVRMEKGIPASPRCGSPTAMEIDKRGSPTPGTRSCLAPSLGLFGASLTMATEVAARVTSTGPHRPQDLALTEPSGRARELEDLQPPEALVERQGQFL Predict reactive species Full Name: inositol 1,4,5-trisphosphate 3-kinase B Calculated Molecular Weight: 946 aa, 102 kDa Observed Molecular Weight: 102-110 kDa GenBank Accession Number: BC015009 Gene Symbol: ITPKB Gene ID (NCBI): 3707 RRID: AB_2129846 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P27987 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.