Proteintech, 12818-1-AP, TAF1B Polyclonal antibody

Size: 20ul / 150ul The TAF1B (12818-1-AP) by Proteintech is a Polyclonal antibody targeting TAF1B in WB, IHC, ELISA applications with reactivity to human samples 12818-1-AP targets TAF1B in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, NIH/3T3 cells Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information TAF1B is a component of the RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. It binds to rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex, and possibly directly through its interaction with RRN3 (PMID: 11250903; 11283244; 15970593; 21921199). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3267 Product name: Recombinant human TAF1B protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-346 aa of BC018137 Sequence: MDLEESEEFKERCTQCAAVSWGLTDEGKYYCTSCHNVTERYQEVTNTDLIPNTQIKALNRGLKKKNNTEKGWDWYVCEGFQYILYQQAEALKNLGVGPELKNDVLHNFWKRYLQKSKQAYCKNPVYTTGRKPTVLEDNLSHSDWASEPELLSDVSCPPFLESGAESQSDIHTRKPFPVSKASQSETSVCSGSLDGVEYSQRKEKGIVKMTMPQTLAFCYLSLLWQREAITLSDLLRFVEEDHIPYINAFQHFPEQMKLYGRDRGIFGIESWPDYEDIYKKTIEVGTFLDLPRFPDITEDCYLHPNILCMKYLMEVNLPDEMHSLTCHVVKMTGMGEVDFLTFDPIA Predict reactive species Full Name: TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa Calculated Molecular Weight: 588 aa, 69 kDa Observed Molecular Weight: 69 kDa GenBank Accession Number: BC018137 Gene Symbol: TAF1B Gene ID (NCBI): 9014 RRID: AB_10644160 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q53T94 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.