Proteintech, 12834-1-AP, HDAC6 Polyclonal antibody

Size: 20ul / 150ul The HDAC6 (12834-1-AP) by Proteintech is a Polyclonal antibody targeting HDAC6 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 12834-1-AP targets HDAC6 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, human liver tissue, Calu-1 cells, L02 cells, K-562 cells Positive IP detected in: K-562 cells Positive IHC detected in: human testis tissue, human breast cancer tissue, human lung cancer tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MG132 treated HepG2 cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is a rabbit polyclonal antibody raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa. Specification Tested Reactivity: human Cited Reactivity: human, pig, canine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3572 Product name: Recombinant human HDAC6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 712-1064 aa of BC013737 Sequence: AERMTTREKKVLEAGMGKVTSASFGEESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTSEEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGATLAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQGTTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADSMLMQGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTIQENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAYVHHQALLDVKNIAHQNKFGEDMPHPH Predict reactive species Full Name: histone deacetylase 6 Calculated Molecular Weight: 1063 aa, 114 kDa, 131 kDa Observed Molecular Weight: 160 kDa GenBank Accession Number: BC013737 Gene Symbol: HDAC6 Gene ID (NCBI): 10013 RRID: AB_10597094 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UBN7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.