Proteintech, 12875-1-AP, PPEF1 Polyclonal antibody

Size: 20ul / 150ul The PPEF1 (12875-1-AP) by Proteintech is a Polyclonal antibody targeting PPEF1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 12875-1-AP targets PPEF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, HEK-293 cells, PC-3 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3553 Product name: Recombinant human PPEF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC036026 Sequence: MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEYADEQGQMQLSTFFSFMLENYTHIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTE Predict reactive species Full Name: protein phosphatase, EF-hand calcium binding domain 1 Calculated Molecular Weight: 653 aa, 76 kDa Observed Molecular Weight: 69-76 kDa GenBank Accession Number: BC036026 Gene Symbol: PPEF1 Gene ID (NCBI): 5475 RRID: AB_2166375 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14829 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.