Proteintech, 12930-1-AP, HRSP12 Polyclonal antibody

Size: 20ul / 150ul The HRSP12 (12930-1-AP) by Proteintech is a Polyclonal antibody targeting HRSP12 in WB, IHC, ELISA applications with reactivity to human, rat samples 12930-1-AP targets HRSP12 in WB, IHC, RIP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: human liver tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Ribonuclease UK114, also named HRSP12, is found predominantly in human adult kidney and liver and is responsible for inhibiting translation by cleaving mRNA. UK114 is mostly expressed in cytoplasm, but occasionally in nucleus in less differentiated cells. UK114 cleaves phosphodiester bonds only in single-stranded RNA. The levels of both the mRNA and protein are markedly reduced in heptatocellular tumors and in human hepatoma cell lines compared with normal liver tissues, suggesting that ribonuclease UK114 may be an important biomarker for heptatic carcinoma. Current antibody catalog# 12930-1-AP is a rabbit polyclonal antibody raised from human full-length UK114. Specification Tested Reactivity: human, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3628 Product name: Recombinant human UK114; HRSP12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-137 aa of BC010280 Sequence: MSSLIRRVISTAKAPGAIGPYSQAVLVDRTIYISGQIGMDPSSGQLVSGGVAEEAKQALKNMGEILKAAGCDFTNVVKTTVLLADINDFNTVNEIYKQYFKSNFPARAAYQVAALPKGSRIEIEAVAIQGPLTTASL Predict reactive species Full Name: heat-responsive protein 12 Calculated Molecular Weight: 14.5 kDa Observed Molecular Weight: 14.5 kDa GenBank Accession Number: BC010280 Gene Symbol: HRSP12 Gene ID (NCBI): 10247 RRID: AB_2119169 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P52758 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.