Proteintech, 12949-1-AP, PDK4 Polyclonal antibody

Size: 20ul / 150ul The PDK4 (12949-1-AP) by Proteintech is a Polyclonal antibody targeting PDK4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 12949-1-AP targets PDK4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, NCI-H1299 cells, mouse heart tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue Positive IHC detected in: human lung cancer tissue, human pancreas cancer tissue, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, bovine, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3629 Product name: Recombinant human PDK4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 61-412 aa of BC040239 Sequence: LPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM Predict reactive species Full Name: pyruvate dehydrogenase kinase, isozyme 4 Calculated Molecular Weight: 411 aa, 46 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC040239 Gene Symbol: PDK4 Gene ID (NCBI): 5166 RRID: AB_2161499 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16654 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.